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Objective To observe the protective effect of the traditional Chinese medicine prescription, Jiu Nao Yi Zhi water extract, on human neuroblastoma SH-SY5Y cell line, its effect on expression of insulin signal transduction pathway, and to explore the related mechanisms.Methods SH-SY5Y cells cultured in vitro, were divided into control group, Jiu Nao Yi Zhi No.1 prescription group and No.3 prescription group.The doses were 0.0625 mg/mL, 0.125 mg/mL, 0.25 mg/mL, 0.5 mg/mL and 1 mg/mL.The thiazolyl blue ( MTT) metabolic rate of each group was determined.The dose of 0.125 mg/mL was chosen for cell immunofluorescence analysis, and to observe the expression of insulin receptor substrates-1 ( IRS-1 ) , cAMP response element binding protein ( CREB ) , and the factors of insulin signal transduction pathway.Results Compared with the control group, MTT metabolic rates of the Jiu Nao Yi Zhi groups were significantly increased (P<0.05), and the cell morphology was much better in those groups, cell body more plump, well-adherent and neurite extensions were observed.The expressions of IRS-1 and CREB were higher than that in the control group.Conclusions The traditional Chinese medicine prescription Jiu Nao Yi Zhi water extract can protect neurons by promoting nerve cell growth, and improving the expression of IRS-1 and CREB, the factors of insulin signal transduction pathway.
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Importance of central laboratories in hospitals is rising sharply as they provide laboratory support to hospital development in its science researches. The paper introduced the mission and features of the central lab of the hospital, as well as its technical personnel makeup, instrumentation, and rules and regulations. Authors prove in the paper that central labs are playing a key role in hospital science research as its research base.
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Objective To set up a HPLC method for the determination of the contents of notoginsenoside R1,ginsenoside Rg1 and Rb1 in Kangliu pills.Methods It was performed on Kromasil C18(4.6 mm?150 mm,5 ?m),and the mobile phase was acetonitrile-0.05% phosphoric acid with gradient elution system.The flow rate was 1.0 mL/min,the detective wavelength was 203 nm,injection volume was 10 ?L and the column temperature was 30 ℃.Results The liner range of notoginsenoside R1 was 82.8～621 ?g(r=0.999 9) and the average recovery was 100.057%(RSD=0.399 6%).The liner range of ginsenoside Rg1 was 109.8～ 823.5 ?g(r =0.999 6) and the average recovery was 99.248%(RSD=1.004 6%).The liner range of ginsenoside Rb1 was 112.6～844.5 ?g(r=0.999 9) and the average recovery was 99.812%(RSD=1.474 4%).Conclusion The method is simple,accurate and reproducible,and suitable for the content determination of notoginsenoside R1,ginsenoside Rg1 and Rb1 in Kangliu pills.
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AIM:To investigate the effects of aminophylline used early in resuscitation on achievement ratio of resuscitation,the concentrations of plasma norepinephrine(NE),adenosine and nitric oxide(NO),and the levels of cardiac tissue endothelin-1(ET-1)and adenosine in rats with sudden cardiac arrest.METHODS:Sixty SD rats were randomly divided into three groups:operated control(group A),epinephrine treatment(group B),and epinephrine plus aminophylline treatment(group C).Each group had 20 rats.The concentrations of plasma NE,adenosine and NO,and the levels of cardiac tissue ET-1 and adenosine were examined in group A and 30 min after survived in group B and group C.RESULTS:The duration of circulation recovered in group C was less than that in group B,significantly(P0.05).The concentrations of plasma adenosine and NE,and the levels of cardiac tissue ET-1 and adenosine in group B and group C were higher than those in group A significantly(P